Immunotherapy Assessment Core
The Immunotherapy Assessment Core provides cutting-edge technologies and expert support for clinical and translational studies, aiming to dissect mechanisms of immunopathogenesis at the single cell level, and exploring novel disease-specific biomarkers with diagnostic and therapeutic applications.
We provide root to branch services for dissection of the immune response both phenotypically, functionally and transcriptionally at the single cell or population level. Single cell RNAseq (10x Genomics Chromium platform) and multispectral fluorescent tissue imaging of PPFE and frozen samples (Akoya Biosciences Vectra 3) are just two in house technologies available to investigators, providing immune cell (and beyond) characterization at the highest resolution. Services also include consultation, sample preparation, controlled rate freezing and cryopreservation/storage, as well as a comprehensive range of standardized and customizable immunological assays.
Contact Us
Co-Directors
Location
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Immunotherapy Assessment Core
Tarry Building, Room 4-730
300 E. Superior St.
Chicago, IL 60611
Phone
- 312-503-7898
Website
Services & Equipment
Key Services
- Consultation
- Instrument training
- PBMC isolation
- Tissue processing
- Controlled rate freezing
- Sample storage
- Magnetic cell sorting
- 10xGenomics single cell RNAseq 3’ library prep
- TCR and transcriptome RNAseq library prep
- Cryosectioning
- Single-multiplex staining
- Multiparameter FACS staining
- Proliferation/ Cytotoxicity assay
- Multiplex (Vectra) imaging
- MSD cytokine analysis
Equipment
- Leica BondRx
- 10X Genomics Chromium System
- Controlled Rate Freezer
- Vectra Multispectral Imager
- Meso Quickplex SQ 120
- Biosafety Cabinet
- Incubator
- Cryogenic Storage Vessel
- Ultra Low Freezer
- Centrifuge
- Water Purifier
- Octo Tissue Dissociator
Acknowledgement
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
[Description of work] was performed by the Immunotherapy Assessment Core at Northwestern University.Core Navigator
For guidance on which cores may be most useful for your research and to coordinate use, please use the Core Navigator chatbot or contact Sara Fernandez Dunne at s-fernandez@northwestern.edu or 847.491.5960.